Mannose and xylose cannot be used as selectable agents for <i>Vitis vinifera</i> L. transformation

Authors

  • F. Kieffer
  • C. Triouleyre
  • C. Bertsch
  • S. Farine
  • Y. Leva
  • B. Walter

DOI:

https://doi.org/10.5073/vitis.2004.43.35-39

Keywords:

mannose, xylose, carbohydrate source, selectable agent, somatic embryogenesis, Vitis vinifera

Abstract

Only a few selectable marker systems for genetic engineering of grapevine have been studied in the past and only resistance to antibiotics has been used to recover transgenic vines. Since the acceptance of antibiotic resistances is small mannose and xylose were evaluated as selectable agents for the genetic transformation of grapevine. Survival of suspension cells and the ability to develop embryos from embryogenic calli were estimated in the presence of mannose and xylose. Embryogenic calli obtained from anther cultures of Vitis vinifera cv. Chardonnay were able to grow and to produce embryos even if mannose or xylose were the only source of carbohydrates.


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Published

2015-04-20

Issue

Vol. 43 No. 1 (2004): Vitis

Section

Article

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