Ripening-related gene expression during fruit ripening in <i>Vitis vinifera</i> L. cv. Cabernet Sauvignon and Clairette blanche

Authors

  • A. L. Burger
  • F. C. Botha

DOI:

https://doi.org/10.5073/vitis.2004.43.59-64

Keywords:

grapevine, ripening-related, gene expression, cDNA-AFLP, fruit ripening, fruit quality

Abstract

The gene expression patterns in ripening fruit of a high quality wine cultivar (Cabernet Sauvignon) and a poor quality wine cultivar (Clairette blanche) were studied using cDNA-AFLP fingerprinting. Total RNA from "immature" (14-weeks post flowering) and "mature" (18-weeks post flowering) berries were used to study ripening-related gene expression in post-véraison stages of berry development. A total of 1,276 fragments were analysed, of which 175 appeared to be ripening-related. Average pairwise differences of the fragments amplified from "immature" and "mature" Cabernet Sauvignon and Clairette blanche berries, revealed a high level of similarity between the two cultivars. 70 % of the ripening-related fragments were cultivar-specific. The number of cultivar-specific and/or ripening-related fragments amplified, depended on the selective nucleotides of the primers used in the cDNA-AFLP analysis. Reverse slot blot and northern blot analysis confirmed that the expression of the identified genes were ripening-related.

 

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Published

2015-04-20

Issue

Vol. 43 No. 2 (2004): Vitis

Section

Article

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