Sequence analysis of a microsatellite and its flanking regions in intraspecific hybrids of grapevine (<i>Vitis vinifera</i> L.)

Authors

  • E. Masi
  • R. Vignani
  • E. Sensi
  • M. Scali
  • M. Cresti
  • M. Biselli

DOI:

https://doi.org/10.5073/vitis.2004.43.69-73

Keywords:

MS polymorphism, DNA, sequence, Vitis vinifera

Abstract

Microsatellite (MS) VVMD21 (BOWERS et al. 1999) was taken as a model to explore the molecular basis of polymorphism in a panel of 6 grapevine accessions (Vitis vinifera L.), consisting of Sangiovese and Cabernet Sauvignon and 4 F1 plants derived from crossing both varieties. The 12 alleles of both parents and the progeny were cloned and sequenced. The microsatellite repeat (AG)n>6 was found in each sequence, together with a poly-T rich region that showed irregularity. Furthermore, single nucleotide deletion or exchange (point mutations) were found in the microsatellite flanking regions.


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Published

2015-04-20

Issue

Vol. 43 No. 2 (2004): Vitis

Section

Article

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