Selection of lactic bacteria to induce malolactic fermentation in red wine of cv. Cencibel
DOI:
https://doi.org/10.5073/vitis.2004.43.149-153Keywords:
lactic bacteria, red wine, selectionAbstract
This paper describes the procedure for the selection of three lactic acid bacteria strains from 40 indigenous strains isolated in two cellars of theSpanish region of Castilla-LaMancha.The isolates were identified by classical microbiological and molecular techniques: Pulsed-Field Gel Electrophoresis (pFGE) of bacterial chromosome fragments obtained from restriction enzymes.
Selection was carried out considering the capacity to perform malolactic fermentation (MLF) in wine and the resistance to high alcoholic percentages and low pH levels. Three isolates (two Lactobacillus planta Tum and one Oenococcus oeni) which were able to pass the above mentioned tests were selected. These isolates produced low volatile acidity, showed a moderate resistance to SO2 and did not cause any degradation of residual sugars.
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