Development and characterization of new microsatellite markers for grape

Authors

  • R. Arroyo-García
  • J. M. Martínez-Zapater

DOI:

https://doi.org/10.5073/vitis.2004.43.175-178

Keywords:

grape, Vitis vinifera, microsatellites, DNA polymorphism

Abstract

Thirty five new grape microsatellite markers were developed under an international consortium involving AGROGENE. These loci were amplified in 41 Spanish cultivars of V. vinifera. Eleven of the markers were polymorphic and informative in V. vinifera. Twelve were monomorphic and of the remaining markers one was polymorphic but less useful because individuals amplified more than two bands and the rest had amplification problems. The number of alleles detected for the 11 informative markers ranged from 4 to 12, with heterozygosity values ranging from 0.6 to 0.8. Primer sequences are reported for these markers.

 

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Published

2015-04-21

Issue

Vol. 43 No. 4 (2004): Vitis

Section

Article

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