Opines in naturally infected grapevine crown gall tumors

Authors

  • E. Szegedi

DOI:

https://doi.org/10.5073/vitis.2003.42.39-41

Keywords:

Agrobacterium vitis, Vitis vinifera, octopine, nopaline, vitopine, polymerase chain reaction

Abstract

Crown gall tumors collected from naturally infected plants of 7 grapevine varieties were analysed for the presence of opines. Eighty-five of the tested 90 samples contained known Agrobacterium vitis-induced opines. Octopine was the most common, it was found in 50 samples. Twentyeight crown galls contained nopaline and 8 were vitopine positive. There was only one tumor that contained two opines, nopaline and vitopine. Five samples were negative for the opines tested. The presence of A. vitis was confirmed in most of these tumors by PCR analysis. The opine assay may provide a simple diagnostic protocol to distinguish healthy callus tissues from crown galls as well as for the indirect identification of Agrobacterium infection.

 

Downloads

Published

2015-04-21

Issue

Vol. 42 No. 1 (2003): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.