Somatic embryogenesis from filaments of <i>Vitis vinifera</i> L. and <i>Vitis labruscana</i> Bailey
DOI:
https://doi.org/10.5073/vitis.2003.42.53-54Keywords:
somatic embryogenesis, filament culture, VitisAbstract
Research Note
Somatic embryogenesis from filaments of grape was investigated. The combination of 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 μM N-(1,2,3-thiadiazol-5-yl)-N’-phenylurea (TDZ) or 10 μM 2,4-D and 10 μM TDZ was suitable for somatic embryogenesis from filaments. The filaments of 8 grape cultivars including recalcitrant genotypes were cultured on a half strength MS basal medium supplemented with 1 μM 2,4-D and 1 μM TDZ. Two-step culture (liquid medium followed by solid medium) gave better results for somatic embryogenesis than one-step culture (solid medium only). Induction of embryogenic callus (EC) was achieved with 5 of 8 cultivars including recalcitrant ones by the two-step culture method, indicating that the filament culture enlarges the number of the EC inducible cultivars.
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