Effect of <i>Agrobacterium rhizogenes<i> infection on in vitro rooting of <i>Vitis vinifera</i>
DOI:
https://doi.org/10.5073/vitis.2003.42.159-161Keywords:
Vitis vinifera, Agrobacterium rhizogenes, rooting, in vitro cultureAbstract
Agrobacterium rhizogenes is known to induce the proliferation of rapid growing, highly branching roots (hairy roots) in most dicotyledonous plants. We report here the effect of in vitro infection of Vitis vinifera with two A. rhizogenes strains (b-glucuronidase transgenic A4 and wild type LBA), with regard to an increase of root mass.
In vitro-grown V. vinifera explants were infected with two A. rhizogenes strains. Both strains induced a significant increase in the number of the developed roots, and of their weight and length. Root number was increased by a factor of 2.9 (strain A4) and 2.7 (strain LBA), length increased 1.9 and 1.6 times (strains A4 and LBA, respectively), while the root weight was more affected by strain A4 (2.8 times increasing) than by LBA strain (1.9 times increasing).
The transformation status of the developed roots was assessed by two different methods: PCR detection of rolB gene in LBA strain-derived roots and quantification of â-glucuronidase activity in A4 strain-derived roots.
Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.