Micropropagation of <i>Vitis amurensis</i> Rupr.: An improved protocol

Authors

  • D. S. Han
  • Y. Niimi
  • J. Y. Wu

DOI:

https://doi.org/10.5073/vitis.2003.42.163-164

Keywords:

in vitro propagation, axillary bud, BA, auxin, sucrose

Abstract

Research Note

An efficient micropropagation procedure of V. amurensis cv. Zuoshan 1 was established. NAA combined with BA resulted in callus formation and inhibition of shoot growth, whereas a combination of 0.3 M IAA and 4.4 M BA gave highest shoot growth and multiplication. IAA at 2.8 and 5.7 M led to high root formation of shoots. 30 g l-1 sucrose was needed for high shoot growth, while high rooting was achieved with 0-20 g l-1 sucrose. Intact leaves are required for a high level of shoot rooting.

 

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Published

2015-04-21

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