Molecular identification of a phytoplasma infecting grapevine in the Republic of Macedonia

Authors

  • M. Šeruga
  • D. Škorić
  • B. Kozina
  • S. Mitrev
  • M. Krajačić
  • M. Ćurković Perica

DOI:

https://doi.org/10.5073/vitis.2003.42.181-184

Keywords:

grapevine yellows, PCR, RFLP, stolbur, tuf, Vitis vinifera L., 16S rDNA

Abstract

Phytoplasmas from the ribosomal subgroup 16SrXII-A (stolbur) were identified by PCR and RFLP analyses of 16S rRNA gene and elongation factor-Tu phytoplasma gene (tuf) in symptomatic grapevines from the Veles and Skopje areas in Macedonia. Two methods were used for nucleic acid extraction. Amplification and RFLP results of 16S rDNA were not influenced by the method used, but RFLP patterns of tuf gene revealed additional bands if a less time consuming method was applied. The possible origin of these bands is discussed. This is the first report of the presence of the stolbur phytoplasma in cvs Chardonnay and Vranac in Macedonia.

 

Downloads

Published

2015-04-21

Issue

Vol. 42 No. 4 (2003): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.