High efficiency somatic embryogenesis and plant germination in grapevine cultivars Chardonnay and Brachetto a grappolo lungo
DOI:
https://doi.org/10.5073/vitis.2001.40.111-115Keywords:
Brachetto, Chardonnay, floral explants, organogenesis, somatic embryogenesisAbstract
A highly efficient, reproducible method for somatic embryogenesis induction, plant recovery and embryogenic culture preservation has been developed for cvs Chardonnay and Brachetto a grappolo lungo (Vitis vinifera), starting from immature anthers and ovaries. Embryogenic induction efficiency was 2 % and 17 % in anthers for Chardonnay and Brachetto g.l., respectively, and 14 % in ovaries for both cultivars. Embryogenic cultures of both genotypes are still propagating 3.5 years after the initial induction and are still morphogenic. Embryo conversion into plantlets occurred at suitable efficiencies during a 100 d culture for both Chardonnay (37 % and 15 %) and Brachetto g.l. (30 % and 29 %), in the two media tested. Organogenesis was also obtained from cotyledonary leaves of Chardonnay.
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