Improved control of water loss from micropropagated grapevines (<i>Vitis vinifera</i> cv. Nebbiolo)
DOI:
https://doi.org/10.5073/vitis.2001.40.137-140Keywords:
grape, micropropagation, acclimatization, hardening, stomata, PBZAbstract
Micropropagated plantlets are generally susceptible to rapid desiccation when exposed to reduced relative humidity and require a costly acclimatization procedure. Detached leaves of micropropagated Vitis vinifera, cv. Nebbiolo, plantlets were used to evaluate the relative contribution of leaf cuticle and stomata to water loss. Water loss occurred mainly from the abaxial surface of detached leaves; moreover, a large majority of stomata was still open 3 h after exposure to 63 % RH. An indirect estimation of epicuticular wax suggested a lower wax deposition for micropropagated plantlets compared to acclimatized and field-grown plants of the same clone. A previously developed method to produce hardened micropropagated plants was adopted: 1 mg(.)l(-1) paclobutrazol (PBZ) was added to the medium and culture vessels with reduced relative humidity (rRH) were used during the last stage of micropropagation. Under our experimental conditions, rRH was more effective to reduce transpiration than PBZ; a combination of both treatments improved plant survival during acclimatization.
Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.