Assessment of the genetic diversity of some important grape genotypes in India using RAPD markers

Authors

  • S. A. Tamhankar
  • S. G. Patil
  • V. S. Rao

DOI:

https://doi.org/10.5073/vitis.2001.40.157-161

Keywords:

genetic diversity, grapes, RAPD, Vitis, India

Abstract

Genetic relationships in a set of important grape genotypes in India, comprising cultivated varieties, rootstocks and wild species were analysed using RAPD markers. A total of 250 bands were obtained by 19 informative primers, most of which could clearly distinguish between the wild and cultivated genotypes. Wild species and rootstocks showed a maximum number of bands (205) and the highest polymorphism (94 %), followed by cultivars belonging to Vitis vinifera (165 bands, 90 %), while cultivars from V. labrusca showed only 75 bands of which almost all were monomorphic. Cluster analysis resulted in the formation of three main clusters. Wild species and rootstocks separated early from cultivated genotypes. Cultivated types formed two separate clusters one consisting mainly of the labrusca and the other of the vinifera types. In the cluster of vinifera genotypes, seeded and seedless varieties were further separated into different subgroups. High bootstrap values at most of the nodes supported the stability of the dendrogram. The grouping of most varieties agreed well with previous reports based on morphological characters as well as parentage, emphasizing the suitability of RAPD analysis for such studies. The present report is the first attempt to determine the genetic relationships in important grape genotypes in India using molecular markers.

 

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Published

2015-05-04

Issue

Vol. 40 No. 3 (2001): Vitis

Section

Article

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