Molecular analysis of fruit ripening: The identification of differentially expressed sequences in <i>Vitis vinifera</i> using cDNA-AFLP technology
DOI:
https://doi.org/10.5073/vitis.2001.40.191-196Keywords:
differential expression, cDNA-AFLP, berry ripening, Vitis viniferaAbstract
Differential gene expression patterns were studied during the ripening process of grape berries (Vitis vinifera cv. Chardonnay). Thirty PstI + MseI primer combinations were used to generate 213 fragments that appeared to be differentially expressed of which 94 % were successfully re-amplified. Reverse northern dot-blot analysis indicated that 35 % of the fragments had similar gene expression profiles to cDNA-AFLPs regarding developmental-stage specificity. Northern blot analyses confirmed the tissue and/or developmental stage specific expression of three of these cDNA fragments. This work illustrates that developmentally regulated sequences can be identified from grape berry tissue using cDNA-AFLP technology.
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