Distribution of phytoplasma in grapevines in the Golan Heights, Israel, and development of a new universal primer

Authors

  • S. Orenstein
  • T. Zahavi
  • P. Weintraub

DOI:

https://doi.org/10.5073/vitis.2001.40.219-223

Keywords:

phytoplasma, stolbur, aster yellows, westem-X, diagnosis, PCR, RFLP

Abstract

Our survey, made in 1999 and 2000, sampled 4 of the most prevalent grapevine cultivars in northern, central and southern regions of the Golan Heights. There were significant differences in the levels of phytoplasma infection between different sub-regions of the Golan Heights; highest levels were found in the south, followed by the center, and the lowest levels in the north. Stolbur was found to be the predominant phytoplasma (~70 %), although aster yellow (~11 %), western-X (~5 %) and mixtures of two phytoplasmas (13 % of all infections, 90 % of which involved Stol and AY) were also found. Prior research on phytoplasmas of grapevines in Israel involved the use of two sets of universal primers. Primers were developed specifically for phytoplasmas occurring in Israel in which there were homologies of 90,85 and 78 % for Stol,AY and W-X phytoplasmas, respectively.

 

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Published

2015-05-05

Issue

Vol. 40 No. 4 (2001): Vitis

Section

Article

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