Detection of a closteroviruslike particle from a corky bark-affected grapevine cultivar

Authors

  • P. L. Monette
  • D. James

DOI:

https://doi.org/10.5073/vitis.1991.30.37-43

Keywords:

virosis, corky bark, closterovirus, test plant, tissue culture, serology, ISEM

Abstract

Closteroviruslike particles were mechanically transmitted from in vitro shoot tip cultures of a grapevine affected with corky bark disease to seedlings of Nicotiana benthamiana. Local necrotic lesions appeared 9-12 d and systemic symptoms 14-16 d post-inoculation. This virus had a normal length of 725 nm, striations with a pitch of 3.4 nm and was 10 nm wide. In ISEM tests, the virus was not decorated with antibodies prepared against GVA, NY-1, CA-4, GLRV-1, GLRV-3, ASGV, PVT or CLSV.

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Published

2015-10-22

Issue

Vol. 30 No. 1 (1991): Vitis

Section

Article

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