Influence of culture technique and genotype on the efficiency of <i>Agrobacterium</i>-mediated transformation of somatic embryos (<i>Vitis vinifera</i>) and their conversion to transgenic plants

Authors

  • Margit Harst
  • Beatrix-Axinja Bornhoff
  • Eva Zyprian
  • R. Töpfer

DOI:

https://doi.org/10.5073/vitis.2000.39.99-102

Keywords:

Agrobacterium tumefaciens, somatic embryogenesis, suspension culture

Abstract

Somatic embryos originating from anther cultures of Vitis vinifera cvs Dornfelder, Muller-Thurgau and Riesling cultured on solid or in liquid medium were used for transformation via Agrobacterium tumefaciens strain LBA 4404. After two days of cocultivation the embryos proliferated in liquid NN69 medium containing kanamycin for selection and cefotaxim for removal of bacteria. Subsequent cultivation of larger embryos was carried out on solid NN69 medium supplemented with kanamycin. Highest conversion rates of selected germinated embryos to rooted plantlets were obtained from embryos of suspension cultures of Dornfelder and Riesling. Transgenic plants of both varieties were adapted to glasshouse and field conditions.

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Published

2015-06-15

Issue

Vol. 39 No. 3 (2000): Vitis

Section

Article

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