Characterization of genetic variation within table grape varieties (<i>Vitis vinifera</i> L.) based on AFLP markers

Authors

  • M. T. Cervera
  • J. A. Cabezas
  • E. Sánchez-Escribano
  • J. L. Cenis
  • J. M. Martínez-Zapater

DOI:

https://doi.org/10.5073/vitis.2000.39.109-114

Keywords:

DNA fingerprinting, molecular markers, variety identification, clone identification, table grape

Abstract

AFLPs were used to characterize a collection of 35 table grape varieties. A single AFLP reaction with a single combination of selective primers yielded an average of 50 polymorphic and scorable amplified bands in this collection and was enough to unequivocally identify each analyzed variety, Genetic similarity between different varieties was estimated, based on the results obtained with two primer combinations, to be between 0.65 and 0.90, while sibling varieties derived from the same cross showed a genetic similarity higher than 0.80, The analysis of several samples belonging to the same varieties, yet from different origins, showed stability of the AFLP patterns as to unequivocally and reproducibly identify them. Furthermore, some specific primer combinations detected a low level of variation that could be due to somatic variation and would be useful in clonal identification.

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Published

2015-06-15

Issue

Vol. 39 No. 3 (2000): Vitis

Section

Article

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