Studies on the genetic diversity of primary and secondary infections of Plasmopara viticola using RAPD/PCR

Authors

  • M. Stark-Urnau
  • M. Seidel
  • W. K. Kast
  • A. R. Gemmrich

DOI:

https://doi.org/10.5073/vitis.2000.39.163-166

Keywords:

downy mildew, grapevine, RAPD/PCR, fingerprint pattern

Abstract

In 1998 and 1999 isolates from primary and secondary infections of Plasmopara viticola (downy mildew) were sampled from several grapevine varieties. Fungal isolates were taken from the same vineyard located in Wurttemberg/Germany. Using the random amplified polymorphic DNA (RAPD) technique as an initial screening for genetic variation within P. viticola isolates, different molecular fingerprint patterns were obtained. Out of 48 isolates of primary infections 27 (1998) and 29 (1999) different genotypes were found. In contrast, variability of secondary infections was lower: about 9 in 1998 and 14 in 1999 different genotypes out of 48 isolates. The high genotype diversity in primary infections and the low level of genotype diversity in secondary infections strongly suggests environmentally directed selection.

Downloads

Published

2015-06-16

Issue

Vol. 39 No. 4 (2000): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.