Influence of some factors on autolysis of <i>Oenococcus oeni</i>
DOI:
https://doi.org/10.5073/vitis.2000.39.167-171Keywords:
Oenococcus oeni, autolysis, N-acetyl-beta-D-glucosaminidaseAbstract
Oenococcus oeni was able to autolyse after transfer to water at 40 degreesC. Cells harvested in the mid-log phase autolyse more rapidly than in other growth phases. Optimum conditions of pH (broad scale,vith two maxima at 4.0 and 7.0) and temperature (40 degreesC) have also been elucidated. Trypsin and EDTA appeared to enhance cell wall autolysis. The activity of several enzymes, namely an amidase or endopeptidase and a N-acetyl-β-D-glucosaminidase, were shown to be involved in the autolytic process, thereby releasing soluble cell wall fragments and nitrogen compounds.Downloads
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