Ultrastructural changes produced in plantlet leaves and protoplasts of <i>Vitis vinifera</i> cv. Cabernet Sauvignon by eutypine, a toxin from <i>Eutypa lata</i>

Authors

  • Corine Deswarte
  • R. Rouquier
  • J. P. Roustan
  • R. Dargent
  • J. Fallot

DOI:

https://doi.org/10.5073/vitis.1994.33.185-188

Keywords:

eutypiosis, eutypine, toxin, Eutypa lata, plantlet leaf, protoplast, ultrastructure, Vitis vinifera

Abstract

Eutypine is a toxin produced by Eutypa lata (Pers.: Pr.) Tul., the causal agent of dying-arm disease of the grapevine. Ultrastructural alterations induced by eutypine in leaf cells and protoplasts isolated from plantlets of Vitis vinifera cv. Cabernet Sauvignon, a very sensitive variety cultured in vitro, were observed for the first time by transmission electron microscopy. Eutypine caused early cytoplasm lysis and vesiculation followed by chloroplast swelling with thylakoid dilation. The eutypine-induced alterations of the cellular ultrastructure are similar to those previously described in vivo in the leaves of diseased grapevines. These results confirm that eutypine, synthesized by E. lata mycelium present in the trunk or arms, is involved in symptom expression of eutypiosis in the herbaceous parts of grapevine.

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Published

2015-08-20

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