Ultrastructural changes produced in plantlet leaves and protoplasts of <i>Vitis vinifera</i> cv. Cabernet Sauvignon by eutypine, a toxin from <i>Eutypa lata</i>
DOI:
https://doi.org/10.5073/vitis.1994.33.185-188Keywords:
eutypiosis, eutypine, toxin, Eutypa lata, plantlet leaf, protoplast, ultrastructure, Vitis viniferaAbstract
Eutypine is a toxin produced by Eutypa lata (Pers.: Pr.) Tul., the causal agent of dying-arm disease of the grapevine. Ultrastructural alterations induced by eutypine in leaf cells and protoplasts isolated from plantlets of Vitis vinifera cv. Cabernet Sauvignon, a very sensitive variety cultured in vitro, were observed for the first time by transmission electron microscopy. Eutypine caused early cytoplasm lysis and vesiculation followed by chloroplast swelling with thylakoid dilation. The eutypine-induced alterations of the cellular ultrastructure are similar to those previously described in vivo in the leaves of diseased grapevines. These results confirm that eutypine, synthesized by E. lata mycelium present in the trunk or arms, is involved in symptom expression of eutypiosis in the herbaceous parts of grapevine.Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.