Embryogenic responses of <i>Vitis</i> spp.: Effects of genotype and polyvinylpyrrolidone

  • I. R. S. Passos
  • B. Appezzato-da-Gloria
  • M. L. C. Vieira
Keywords: somatic embryogenesis, Vitis, histology, DL-phenylalanine, polyvinylpyrrolidone


Somatic embryos were obtained from leaf discs excised from cv. Seyve Villard 5276 (Vitis sp.) and from a Brazilian interspecific hybrid derived from Madalena (Vitis sp.) and Magnolia (V. rotundifolia). Experimental procedures were conducted according to HARST (1995) except that phenylalanine was replaced by polyvinylpyrrolidone which improved the embryogenic response of cultivar SV-5276. Standard somatic embryogenesis was confirmed by histological and ultrastructural analysis which also revealed teratological embryonic development. Secondary embryos were observed and documented by scanning microscopy