Growth enhancement of grapevine call us by catechin on auxin-free media
DOI:
https://doi.org/10.5073/vitis.1998.37.67-71Keywords:
grapevine, in vitro culture, callus, flavanols, catechin, growth promotionAbstract
Proliferating Spätburgunder cells cultured in vitro were found to synthesize the monomeric and oligomeric flavanols catechin, C2, B3, B1, B2 prodelphinidin, ECG (epicatechin gallate), B2G, B2-gallate and B5. Catechin and prodelphinidin were the predominating compounds of all calli studied. Growth promotion of calli from Spatburgunder and Romulus was studied using MS media containing benzyladenine (BA) alone (control), BA plus catechin, and BA combined with two different concentrations of indole-3-acetic acid (IAA). Growth on BA alone was notably reduced. Catechin (68 µM) stimulated callus growth several-fold when combined with 0.8 µM BA; however, this effect was only observed with explants excised from internodes of vigorously growing young shoots.Downloads
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