<i>In vitro</i> germination of stenospermic seeds from reciprocal crosses (<i>Vitis vinifera</i> L.) applying different techniques
DOI:
https://doi.org/10.5073/vitis.1997.36.105-107Keywords:
table grape, stenospermic seed, in ovulo culture, embryo rescue, direct germination, seed coat ruptureAbstract
The frequency of germination of stenospermic seeds cultured in vitro is analysed for two crosses of seedless varieties (Vitis vinifera L.) and their reciprocals. In the case of Superior x Dawn (SxD) the frequency of germination was not different to that of Superior x Ruby (SxR). Results of the reciprocal cross (SxD, DxS) show significantly better results with SxD. In the reciprocal cross SxR and RxS direct germination (DG) is compared with embryo excision (EE) and seed coat rupture (SCR). The majority of embryos of varieties with very soft seed coats, e.g. Superior, germinate directly: 18.8 % in DG, 18.4 % in EE and 23.2 % in SCR. Germination of varieties with harder seed coats (Ruby) could be mechanically affected: 17.6 % in DG, 62.4 % in EE and 58.8 % in SCR. There is no significant difference between EE and SCR. The results of SCR confirm that there is no inhibition of in vitro germination by chemical inhibitors in the seed tissues. The results of CR and EE for SxR and RxS show a greater capacity of Ruby to generate viable embryos.Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.