Identification and discrimination of eight Greek grape cultivars (<i>Vitis vinifera</i> L.) by random amplified polymorphic DNA markers

Authors

  • M. N. Stavrakakis
  • K. Biniari
  • P. Hatzopoulos

DOI:

https://doi.org/10.5073/vitis.1997.36.175-178

Keywords:

dendrogram, grape cultivars, PCR, RAPD markers, Vitis vinifera, similarity index

Abstract

Fifteen decamer primers of an arbitrary nucleotide sequence were used to amplify genomic DNA by polymerase chain reaction (PCR-RAPD) in order to identify and discriminate between 8 cultivars of Vitis vinifera L., grown at the Island of Crete. Over 140 reproducible polymorphic fragments were generated by this method. Each grape cultivar showed a unique banding pattern for more than 5 of the primers used. Herefrom, the degree of genetic similarity was calculated and the dendrogram of the 8 cultivars was constructed. The results show that RAPD is a reliable and very useful method for the identification and genomic analysis of grape cultivars.

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Published

2015-08-06

Issue

Vol. 36 No. 4 (1997): Vitis

Section

Article

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