Identification of grapevine rootstock cv. Börner and differentiation of 125 AA from 5 BB and SO 4

Authors

  • F. Bauer
  • Eva Zyprian

DOI:

https://doi.org/10.5073/vitis.1997.36.185-189

Keywords:

rootstock, variety identification, specific PCR primers

Abstract

Grapevine rootstock cultivars were investigated for polymorphic RAPD-PCR amplification products. Variety-specific RAPD markers were cloned and partially sequenced. Based on this information longer, sequence-specific primer pairs for PCR under highly stringent conditions were designed and tested. The primer pair Börner-1 yielded a reproducible and specific product of 589 bp from cv. Börner DNA. The second primer pair, 5C-1, derived from an originally cv. 5 C-specific RAPD band, yielded a more complex, but still polymorphic amplification pattern within the five rootstock varieties studied.

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Published

2015-08-06

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