Evidence for ferredoxin glutamate synthase activity in grape shoot tissues

Authors

  • G. L. Creasy
  • P. J. Breen

DOI:

https://doi.org/10.5073/vitis.1997.36.207-208

Keywords:

Fd-glutamate synthase, ammonium assimilation

Abstract

Research Note

Ferredoxin glutamate synthase (ferredoxin glutamine: oxoglutarate aminotransferase, or Fd-GOGAT, EC 1.4.7.1) activity was detected in grapevine (Vitis vinifera L.). Highest Fd-GOGAT activity was found in lamina tissue. Moderate levels of activity were found in petiole, tendril, rachis, and flower tissues. No activity was detected in extracts from pedicel tissue. Western blotting with rice Fd-GOGAT antibodies resulted in a positive reaction with single grape protein band of 73 kDa in all tissue types tested.

Downloads

Published

2015-08-06

Issue

Vol. 36 No. 4 (1997): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.