Immunodetection of overwintering <i>Oidium</i> mycelium in bud scales of <i>Vitis vinifera</i>
DOI:
https://doi.org/10.5073/vitis.1996.35.63-64Keywords:
Vitis, Oidium, Uncinula necator, immunofluorescenceAbstract
Research Note
Antigens were isolated from the walls of Oidium conidia by a simple extraction method. Polyclonal antiserum was produced against these antigens and an immunofluorescent assay was developed to detect overwintering mycelium of Oidium in dormant buds. The results show that a specific detection of Oidium mycelium is possible without visualizing other fungal mycelia in dormant buds of grapevines.
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