Extraction of DNA from eggs of grape phylloxera (<i>Daktulosphaira vitifoliae</i> FITCH) for use in RAPD testing
DOI:
https://doi.org/10.5073/vitis.1996.35.87-89Keywords:
Vitis, DNA extraction, RAPD, phylloxera, Daktulosphaira vitifoliae, parthenogenesisAbstract
Three different extraction methods for preparing DNA from single grape phylloxera (Daktulosphaira vitifoliae) eggs for use in PCR-RAPD assay were compared. Pulverization in a glass micro-grinder produced much more clear and consistent RAPD results than when the egg DNA was extracted with a Chelex lysis or a proteinase-K+ lysis. Multiple single egg extractions from the same parthenogenetic colony were done with all three methods; no RAPD assay differences were detected. This technique will allow evaluation of the roles population diversity, mutation rates, and reproductive mechanisms play in the Vitis/phylloxera interaction.Downloads
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