Molecular diagnosis of grapevine fleck virus

Authors

  • S. Sabanadzovic
  • P. Saldarelli
  • V. Savino

DOI:

https://doi.org/10.5073/vitis.1996.35.137-140

Keywords:

diagnosis, fleck, asteroid mosaic, molecular hybridization, riboprobes, digoxigenin-mediated chemiluminescence, RT-PCR, dot spot, tissue blot

Abstract

A digoxigenin-labelled riboprobe was developed for the detection of grapevine fleck virus (GFkV) in infected tissues of grapevine leaves, roots and canes. The probe was GFkV-specific and was successfully used for virus identification both with dot spot assays, using alkali-treated crude sap, and tissue blot assays, using cross and longitudinal sections of leaf petioles. Primers designed for the amplification by reverse transcription-polymerase chain reaction of a viral genome fragment 243 nucleotides in size, gave also positive and repeatable results. These newly developed molecular-based detection tools extend the range of available procedures for the sensitive identification of GFkV in naturally infected hosts.

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Published

2015-08-13

Issue

Vol. 35 No. 3 (1996): Vitis

Section

Article

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