Evaluation of microsatellite sequence-tagged site markers for characterizing <i>Vitis vinifera</i> cultivars
DOI:
https://doi.org/10.5073/vitis.1995.34.99-102Keywords:
grapevine, cultivar identification, DNA, microsatellite analysis, STMS, molecular markers, databaseAbstract
Twenty four cultivars and clones from Italian germplasm collections were DNA typed for the four sequence-tagged microsatellite sites VVS1-FP, VVS2-JOE, VVS5-FP and VV29-TAM. The analysis was performed using primers labelled with different fluorochromes and using a GENESCAN apparatus. The results were compared with data obtained for samples from Australian collections. By comparing the combined genotype of the studied loci all the cultivars could be singularly distinguished except Favorita, Pigato and Vermentino, thus supporting the hypothesis that all three are the same cultivar. Cultivars common to Italian and Australian germplasm collections were found to have the same genotype indicating that the study of microsatellites as sequence-tagged site (STS) markers is a suitable universal system for worldwide grapevine cultivar identification. Application of the semi-automated GENESCAN system made the analysis of microsatellite STS markers fast and reproducible between laboratories.Downloads
Published
Issue
Section
License
The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.