PCR-RFLP analysis of <i>Vitis, Ampelopsis</i> and <i>Parthenocissus</i> and its application to the identification of rootstocks

Authors

  • J. C. Bourquin
  • L. Otten
  • B. Walter

DOI:

https://doi.org/10.5073/vitis.1995.34.103-108

Keywords:

RFLP, Vitis, rootstock identification

Abstract

Methodologies based on the analysis of DNA polymorphism were recently described to identify and study grapevine rootstocks and cultivars. We report here rapid RFLP analysis of 17 Vitis, 3 Ampelopsis and 2 Parthenocissus DNAs amplified by PCR with primers based on sequences from previously defined polymorphic DNA fragments of V. vinifera cv. Chardonnay. These primers can be used as a tool for phylogenetic studies in the genus Vitis and more generally within the Vitaceae family. The DNAs of 22 rootstocks could be identified by PCR using 4 pairs of Chardonnay-derived primers combined with RFLP analysis. However, it was impossible to discriminate between 9 clones of the rootstock 3309 C. This technique is rapid and well reproducible.

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Published

2015-08-14

Issue

Vol. 34 No. 2 (1995): Vitis

Section

Article

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