Peroxidase isoenzymes as markers of cell de-differentiation in grapevines (<i>Vitis vinifera</i>)
DOI:
https://doi.org/10.5073/vitis.1995.34.207-210Keywords:
grapevine, cell cultures, cell de-differentiation, peroxidase, isoenzyme expression, subcellular localizationAbstract
The expression, and the tissue localization and the subcellular localization of peroxidase isoenzymes were studied during the development of mesocarp and hypodermal tissues of Vitis vinifera fruits cvs Airen and Monastrell until fruit softening. In addition the de-differentiation process of mesocarp tissues to form callus cultures was investigated. Both grapevine cultivars contain, as the only component of peroxidase polymorphism in the whole fruit, the peroxidase isoenzyme Bg (the sole component of the peroxidase isoenzyme group HpI BPrx in grapevines), which is both developmentally-regulated and tissue-specific. The establishment of cell cultures from the mesocarp of grapevine fruits is accompanied by the de novo expression of one acidic (APrx) and one basic (LpI BPrx) peroxidase isoenzyme group, whose particular isoenzyme composition is cultivar-specific and dependent on subcellular location (soluble or bound to cell wall). These results suggest that, during the establishment of grape vine cell cultures from mesocarp tissues of grapevine fruits, there is a differential expression of the peroxidase isoenzyme groups APrx and LpI BPrx located in both soluble and cell wall-bound fractions. This de novo gene expression probably expresses at molecular level the totipotency of grapevine somatic cells, which is linked to the de-differentiation process associated with the in vitro culture.Downloads
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