Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)

Authors

  • Z. Marković University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Svetišimunska 15, 10000 Zagreb, Croatia
  • D. Preiner University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb, Croatia
  • D. Stupić University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb, Croatia
  • Ž. Andabaka University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb, Croatia
  • S. Šimon University of Zagreb, Faculty of Agriculture, Department of Plant Breeding, Genetics and Biometrics, Zagreb, Croatia
  • D. Vončina University of Zagreb, Department of Plant Pathology, Zagreb, Croatia
  • E. Maletić University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb, Croatia
  • J. Karoglan Kontić University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb, Croatia
  • P. Chatelet INRA, UMR AGAP, Equipe DAAV, Montpellier, France
  • F. Engelmann IRD, UMR DIADE, Montpellier, France

DOI:

https://doi.org/10.5073/vitis.2015.54.special-issue.247-251

Keywords:

grapevine, cryopreservation, cryotherapy, genetic stability

Abstract

This study aimed at testing the efficiency of a droplet-vitrification cryopreservation protocol in eliminating selected grapevine viruses. The cryopreservation protocol led to approximately 50 % recovery with cultivar 'Portan' and five international cultivars tested, but very low recovery was noted with Croatian cultivars. GFLV and GLRaV-3, two (economically important grapevine) viruses were eliminated in a high percentage (up to 100 %) of plants regenerated from cryopreserved shoot tips. Virus sanitation was observed as well in samples before liquid nitrogen exposure. Genetic stability of plants regenerated after cryopreservation was studied using AFLP markers. Polymorphic fragments were observed in non-cryopreserved and cryopreserved samples treated with PVS2 solution, the number of which increased with increasing durations of exposure to PVS2 solution.

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Published

2015-08-17

Issue

Vol. 54 (2015): Vitis (Special Issue)

Section

Article

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