Micropropagation of grapevine (Vitis vinifera L.) seedless cultivar 'Parvana' through lateral bud development

Authors

  • G. Melyan Scientific Center of Agrobiotechnology, ANAU, 1 Isi-Le-Mulino Str., 1101 Echmiadzin, Armenia
  • A. Sahakyan Scientific Center of Agrobiotechnology, ANAU, Echmiadzin, Armenia
  • A. Harutyunyan Armenian National Agrarian University, Yerevan, Armenia

DOI:

https://doi.org/10.5073/vitis.2015.54.special-issue.253-255

Keywords:

in vitro, micropropagation, explants, grapevine, plant regeneration, lateral bud

Abstract

Factors affecting successful establishment in vitro, rapid proliferation, rooting and acclimatization of grapevine seedless cultivation 'Parvana' were studied. A treatment combination of 2.0 % calcium hypochlorite for 7 minutes plus 70 % ethanol for 3 minutes was found to be effective with 75.0 % of aseptic culture establishment in vitro. The highest number of new microshoots (4.5) was obtained in a culture medium (Gambourg's B5 modification) supplemented with 0.6 mg∙L-1 BAP + 0.2 mg∙L-1 KIN + 0.5 mg∙L-1 GA3. Presence of GA3 in combination with BAP and KIN promoted elongation of shoots. A concentration of 0.4 mg/l IAA in half strength of MS (Murashige and Skoog) proved to be the best for rooting (84.4 % root induction, 4.66 roots with 7.9 cm length). In vitro rooted plantlets were successfully acclimatized, with 82.2 % survival rate in plastic pots containing garden soil, sand and peat moss(1:1:1). In this study an efficient micro propagation technique of grapevine seedless cultivar 'Parvana' was successfully achieved through lateral bud culture. Such a technique would be useful for large scale plant production and in vitro conservation.

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Published

2015-08-17

Issue

Vol. 54 (2015): Vitis (Special Issue)

Section

Article

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