Localisation des tanins dans la pellicule de baie de raisin

Authors

  • K. Amrani Joutei
  • Y. Glories
  • M. Mercier

DOI:

https://doi.org/10.5073/vitis.1994.33.133-138

Keywords:

tannins, localization, ultrastructure, microscopy, berry skin

Abstract

La localisation des taoins dans Ja pellicule de raisin a été étudiée en combinant les resultats obtenus par deux méthodes: observation microscopique (photonique et électronique à  transmission), et analyse chimique de différentes fractions obtenues après isolement des cellules pelliculaires sous forme de protoplastes. Trois grands groupes de tanins ont ainsi été déterminées selon leur localisation: tanins libres dans le suc vacuolaire, visualisés sous forme de granules qui augmentent de taille au fur et à mesure que l'on se rapproche de la surface de la pellicule; des tanins liés ont été mis en évidence dans deux autres localisations: tanins liés aux polysaccharides des parois cellulaires par des liaisons osidiques et tanins liés avec les protéines constitutives de la face interne des membranes vacuolaires.

Localization of tannins in grape berry skins

The localization of tannins in grape berry skins has been studied by associating results obtained with two methods: light and transmission electron microscopy, and chemical analysis of the fractions obtained after isolation of cells as protoplasts. According to their location, three groups of tannins were determined. The first consists of free tannins in solution in the vacuolar sap (these tannins appear in the form of granules decreasing in size from the skin surface to the pulp). Two other groups are constituted by bound tannins: those bound to the proteins of the internal face of the tonoplast, and those bound to the cell wall polysaccharides by osidic bonds.

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Published

2015-08-20

Issue

Vol. 33 No. 3 (1994): Vitis

Section

Article

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