Peroxidase isoenzyme patterns in Vitaceae

Authors

  • O. Bachmann

DOI:

https://doi.org/10.5073/vitis.1994.33.151-156

Keywords:

peroxidase isoenzyme, interspecific cultivars, Vitis species, ploidy

Abstract

The isolation of active cytoplasmatic enzymes has been simplified and improved. Employing this technique 313 different cultivars and species of Vitis have been analyzed for peroxidase isoenzymes in the phloem of dormant canes. Using isoelectric focusing on polyacrylamid gel layers 38 different banding patterns were obtained. Bands with isoelectric points pH 3.5 to 3.8 (type A) were found to be characteristic for Vitis vinifera cultivars (211 samples). The frequency of type A in other Vitis species is 5%. Only half of the interspecific cvs tested showed a type A pattern. Variability in neutral to basic peroxidases was useful to group V. vinifera and interspecific cultivars. The ploidy had no influence on the patterns whereas different colour variants exhibited differences. The single polymorphic isoenzyme peroxidase is sufficient to group cultivars and to discriminate between two samples.

Downloads

Published

2015-08-20

Issue

Vol. 33 No. 3 (1994): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.