Application of on-line coupled mass spectrometric techniques for the study of isomeric vitispiranes and their precursors of grapevine cv. Riesling

Authors

  • G. Full
  • P. Winterhalter

DOI:

https://doi.org/10.5073/vitis.1994.33.241-244

Keywords:

vitispiranes, enantiodifferetiation, Riesling wine, glucosidic precursor, leaf

Abstract

On-line coupled multidimensional gas chromatography - mass spectrometfy (MDGC-MS) in the column combination DB-Wax/2,6-di-O-methyl-3-O-pentyl-β-cyclo-dextrin allowed for the first time the determination of the enantiomeric composition of vitispiranes in Riesling wine. The order of elution was determined with the help of optically pure reference compounds. In the case of the (2R,5R)/(2S,5S)-enantiomers almost racemic proportions were determined, whereas in the case of the (2R,5S)/(2S,5R)-enantiomers the (2S,SR)-isomer prevailed. On-line coupled liquid chromatography - tandem mass spectrometry (LC-MS/MS) furthermore allowed the identification of the vitisprane-yielding precursor 3,4-dihydroxy-7,8-dihydro-β-ionol 3-O-β-D-glucopyranoside in Riesling wine and grapevine leaves.

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Published

2015-08-20

Issue

Vol. 33 No. 4 (1994): Vitis

Section

Article

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