Einfluß verschiedener Vorbehandlungen auf die Induktion somatischer Embryogenese an Antheren der Rebsorte Riesling

Authors

  • Margit Harst-Langenbucher
  • G. Alleweldt

DOI:

https://doi.org/10.5073/vitis.1993.32.1-7

Keywords:

BAP (benzyladenine purine), TDZ (Thidiazuron, N-phneyl-N'-1, 2, 3-thiadiazol-5-yl-urea), callus induction, culture temperature, pretreatment

Abstract

Zur Verbesserung der Regeneration intakter Pflanzen aus Antheren der Rebsorte Riesling wurden verschiedene Vorbehandlungsmaßnahmen am Ausgangsmaterial sowie Variationen in der Subkultivierung der induzierten somatischen Embryonen durchgeführt und dabei folgende Ergebnisse erzielt:

  • Die Kühlung der präparierten Antheren nach Inokulation auf das Nährmedium für 4 d bei 4 °C führte zu einer Regenerationsrate intakter Pflanzen von 8 %.
  • Eine konstante Kulturtemperatur von 27 °C ist zur Induktion von Embryonen optimal; sowohl l5 °C als auch 32 °C hemmten die Embryoinduktion.
  • Die höchsten Embryogeneseraten von 5-7 % wurden nach einer Kallusinduktionsphase von 1-2 Wochen und nachfolgender Kultivierung mit 10 µM BAP oder TDZ erzielt.

The effect of different pretreatments on induction of somatic embryogenesis on anthers of grapevine cv. Riesling

Different pretreatments on starting material for anther cultivation of grapevine cv. Riesling have been carried out. The experiments have been focused on chilling (+4°C) and duration (0-4 d) of inflorescences and freshly excised anthers before cultivation. Best results were obtained when anthers were cooled for a period of 4 d. Callus induction temperature and duration as well as the effects of cytokinins (BA-P, TDZ) in subcultures were also tested. The regeneration rate could be increased by a culture temperature of 27°C, a short callus induction period of 1-2 weeks in combination with either a BAP- or a TDZ-application of 10 µM in the subsequent subcultivation.

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Published

2015-09-02

Issue

Vol. 32 No. 1 (1993): Vitis

Section

Article

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