Detection of grapevine fenleaf virus (GFLV) in infected grapevines by non-radioactive nucleic acid hybridisation

Authors

  • A. R. Gemmrich
  • G. Link
  • M. Seidel

DOI:

https://doi.org/10.5073/vitis.1993.32.237-242

Keywords:

Vitis, nepovirus, non-radiactive cDNA probe, diagnosis

Abstract

The nucleic acid hybridisation technique was adopted for the detection of grapevine fanleaf virus (GFLV) in grapevine tissues using a nonradioactive labeled cDNA. In crude plant sap a certain detection was not successful. Thus, a method was developed for the extraction of total RNA from a large number of samples in a microscale. By Northern blot hybridisation and by the more convenient slot blot technique GFLV infections could be detected. Comparing ELISA and slot blot hybridisation assay using identical plant material different results were obtained with some samples. This indicates different detection spectra for both techniques. The hybridisation assay has been found to be a suitable method by which a large number of samples from different grapevine tissues could be efficiently indexed for GFLV.

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Published

2015-09-02

Issue

Vol. 32 No. 4 (1993): Vitis

Section

Article

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