<i>In vivo</i> development of ovule in seedless and seeded cultivars of grapes (<i>Vitis vinifera</i> L.)- a particular reference to <i>in ovulo embryo</i> culture

Authors

  • Zora Singh
  • S. J. S. Brar

DOI:

https://doi.org/10.5073/vitis.1992.31.77-82

Keywords:

table grape, seedlessness, development, berry, ovule, embryo, tissue culture

Abstract

Ovule development was examined in the three seedless cvs Perlette, Thompson Seedless, Beauty Seedless and the seeded cv. Anab-e-Shahi, to identify the proper developmental stage of the embryo for in ovulo embryo culture. The number of shrivelled ovules started increasing 20 d post anthesis. At the same time, the number of viable ovules started declining in all the seedless cultivars. Amongst seedless cultivars, the growth of ovules was least in cvs Thompson Seedless and Beauty Seedless. The ovule development was faster in Anab-e-Shahi as compared to all the seedless cultivars. Total number of ovules per berry declined 20 and 30 d post anthesis in cvs Beauty Seedless and Perlette, respectively. Keeping in view the above mentioned parameters, the embryos in seedless cultivars of grapes may be rescued in vitro prior to 20 d post anthesis to obtain plantlets from these abortive ovules.

Die Entwicklung der Samenanlagen bei kernlosen und kernhaltigen Rebsorten (Vitis vinifera L.) in vivo im Hinblick auf die Embryokultur in vitro

Um das geeignetste Entwicklungsstadium der Embryonen für ihre in-vitro-Kultur zu ermitteln, wurde bei den drei kernlosen Rebsorten Perlette, Thompson Seedless und Beauty Seedless sowie der kernhaltigen Sorte Anab-e-Shahi die Entwicklung der Samenanlagen verfolgt. Die Anzahl geschrumpfter Samenanlagen begann 20 d nach der Anthese anzusteigen. Gleichzeitig setzte bei allen kernlosen Sorten die Abnahme der vitalen Samenanlagen ein. Von den kernlosen Sorten zeigten Thompson Seedless und Beauty Seedless das schwächste Wachstum der Samenanlagen. Bei Anab-e-Shahi entwickelten sich die Samenanlagen schneller als bei den drei kernlosen Sorten. Bei Beauty Seedless und Perlette ging die Gesamtzahl der Samenanlagen 20 bzw. 30 d nach der Anthese zurück. Unter Berücksichtigung der oben genannten Entwicklungszeiten sollten die Embryonen binnen 20 d nach der Anthese in Kultur genommen werden, um aus den später absterbenden Samenanlagen in-vitro-Pflanzen zu gewinnen.

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Published

2015-10-08

Issue

Vol. 31 No. 2 (1992): Vitis

Section

Article

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