The isolation from <i>Saccharomyces cerevisiae</i> of two antibacterial cationic proteins that inhibit malolactic bacteria
DOI:
https://doi.org/10.5073/vitis.1992.31.105-116Keywords:
Saccharomyces cerevisiae, yeast, cationic proteins, antibacterial proteins, bacteria, lactic acid, malo-lactic fermentation, growth inhibitionAbstract
Substances with antibacterial activity were recovered from the extracellular medium and from the acidified cell extract of Saccharomyces cerevisiae R107 by binding onto a cation exchanger. The fraction eluted with NaOH contained two different antibacterial factors, one with the characteristics of lysozyme, the other apparently a small protein with a similar high pI.
Die Isolierung von zwei bakteriziden, kationischen Proteinen aus Saccharomyces cerevisiae, die Bakterien des Säureabbaus hemmen
Substanzen mit bakterizider Wirkung wurden sowohl aus dem Kultursubstrat als auch aus angesäuertem Zellextrakt von Saccharomyces cerevisiae Rl07 mit Hilfe von Kationenaustauscher isoliert. Die mit NaOH eluierte Fraktion enthielt zwei unterschiedliche bakterizide Substanzen, eine mit Lysozymeigenschaften, die andere, ein kleines Protein, mit ähnlich hohem isoelektrischen Punkt.
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