Partial characterization of <i>Agrobacterium vitis</i> strains

Authors

  • S. Matsumoto
  • K. Ophel
  • K. G. M. Skene
  • N. S. Scott

DOI:

https://doi.org/10.5073/vitis.1992.31.195-203

Keywords:

Agrobacterium vitis, Agrobacterium tumefaciens, Vitis vinifera, vir genes

Abstract

Seventeen strains of Agrobacterium vitis (formerly classified A. tumefaciens biovar 3) were characterized using part of the T-DNA and virA regions of the Ti plasmid as probes. All strains except one were of the wide host range (WHR) strains and were classified into two groups depending on their ability to utilize octopine or nopaline. These WHR type oncogenic strains had homology with the limited host range type (LHR) virA gen of A. vitis but not with the WHR virA gene of A. tumefaciens.
The frequency of T-DNA excision in some Agrobacterium strains was estimated with the plasmid pTMA which mimics T-DNA excision from Ti plasmid DNA. In an A. vitis strain isolated from grapevine, T-DNA excision occurred after co-cultivation with grapevine tissues, but not with acetosyringone. In contrast, in A. tumefaciens, T-DNA excision occurred after co-cultivation with acetosyringone, but not with grapevine tissue.

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Published

2015-10-09

Issue

Vol. 31 No. 4 (1992): Vitis

Section

Article

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