Detection of grapevine closterovirus A in infected grapevine tissue by reverse transcription-polymerase chain reaction

Authors

  • A. Minafra
  • A. Hadidi
  • G. P. Martelli

DOI:

https://doi.org/10.5073/vitis.1992.31.221-227

Keywords:

grapevine closterovirus A, cDNA, reverse transcription polymerase chain reaction, molecular diagnosis

Abstract

Reverse transcription-polymerase chain reaction (RT-PCR) was successfully applied to detection of GVA RNA in nucleic acid extracts of infected grapevines. In particular, an artificially synthesized DNA primer set designed to amplify a GVA cDNA fragment of 430 base pairs, specifically detected GVA RNA sequences in extracts from infected grapevine tissues such as leaves from in vitro-grown explants, leaves from greenhouse-grown rooted cuttings, and bark scrapings of mature canes from field-grown vines. The detection limit of GVA RNA by RT-PCR was estimated to be 200 fold higher than that obtained by molecular hybridization or ELISA.

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Published

2015-10-09

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