Etude de la toxicité du filtrat de culture de <i>Botrytis cinerea</i> sur des vitroplants de vignes: <p>I. Effet du filtrat brut</p>
DOI:
https://doi.org/10.5073/vitis.1991.30.167-175Keywords:
Botrytis, Vitis, variety of vine, tissue culture, fungal culture filtrate, toxicity, growth, resistance, test, methodAbstract
Study of culture filtrate toxicity of Botrytis cinerea observed on grapevine vitroplants:
I. Effect of crude culture filtrate
The purpose of this research was:
- to show culture filtrate toxicity of Botrytis cinerea,
- to determine an eventual relation between susceptibility of grapevines to B. cinerea in the field and susceptibility of in vitro plantlets to fungal culture filtrate.
In this way, effects of culture filtrate on the growth of vitroplants were examined to evaluate on the one hand, susceptibility of Vitis and, on the other, degree of culture filtrate toxicity. Growth inhibition increased with grapevine sensitivity and no phytotoxic effects were observed on resistant grapevines. So a discrimination between resistant, susceptible and very susceptible grapevines was achieved. A comparative study has also shown that factors like fungal culture age or isolate nature were able to affect toxicity. 1-week-old culture filtrate was able to induce symptoms on vitroplants and toxicity increased with fungal culture age. Moreover, this was not due to enzyme activity.
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