Caracterización de variedades y portainjertos de vid mediante isoenzimas de los sarmientos

Authors

  • H. Altube
  • F. Cabello
  • J. M. Ortiz

DOI:

https://doi.org/10.5073/vitis.1991.30.203-212

Keywords:

ampelography, chemotaxonomy, variety of vine, rootstock, wood, analysis, enzyme, protein

Abstract

Characterization of grape varieties and rootstocks by isoenzymes from woody parts

22 Vitis vinifera cultivars and 4 rootstocks were chosen for studying of their biochemical characterization, based on the electrophoretical separation of isozymes. 7 isoenzyme systems, including acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), glucose phosphate isomerases (GPI), malate dehydrogenases (MDH) and peroxidases (PER), were extracted from woody tissue, separated in polyacrylamide gels and stained for their observation. ACPH, CO, EST, MDH and PER were the most adequate systems for characterization. The results of the different systems and the grouping of the cultivars are presented. The use of these isozyme techniques in future studies on characterization of Vitis genotypes is suggested.

Downloads

Published

2015-10-22

Issue

Vol. 30 No. 4 (1991): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.