Caracterización de variedades y portainjertos de vid mediante isoenzimas de los sarmientos
DOI:
https://doi.org/10.5073/vitis.1991.30.203-212Keywords:
ampelography, chemotaxonomy, variety of vine, rootstock, wood, analysis, enzyme, proteinAbstract
Characterization of grape varieties and rootstocks by isoenzymes from woody parts
22 Vitis vinifera cultivars and 4 rootstocks were chosen for studying of their biochemical characterization, based on the electrophoretical separation of isozymes. 7 isoenzyme systems, including acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), glucose phosphate isomerases (GPI), malate dehydrogenases (MDH) and peroxidases (PER), were extracted from woody tissue, separated in polyacrylamide gels and stained for their observation. ACPH, CO, EST, MDH and PER were the most adequate systems for characterization. The results of the different systems and the grouping of the cultivars are presented. The use of these isozyme techniques in future studies on characterization of Vitis genotypes is suggested.
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