Etude de la toxicité du filtrat de culture de <i>Botrytis cinerea</i> sur des'vitroplants de vignes: <p>II. Effet des différentes fractions isolées a partir du filtrat brut</p>

Authors

  • D. Vannel
  • M. Barbier
  • R. Bessis

DOI:

https://doi.org/10.5073/vitis.1991.30.213-218

Keywords:

Botrytis, Vitis, variety of vine, tissue culture, fungal culture filtrate, glucan, heteropolysaccharides, toxicity, growth, resistance, test, method

Abstract

Study of culture filtrate toxicity of Botrytis cinerea observed on grapevine vitroplants:
II. Effect of various fractions isolated from the crude culture filtrate

Polysaccharides secreted by Botrytis cinerea were isolated from the crude culture filtrate and precipitated as two fractions: glucan and heteropolysaccharides. The fraction containing heteropolysaccharides was further separated into two sub-fractions. The phytotoxic activities of the various fractions were tested on vitroplants from several grapevines differing in their sensitivity to B. cinerea. With glucan, no toxic effects on the growth of vitroplants were observed. The heteropolysaccharides showed specific phytotoxic activities which were identical with the effects of the culture filtrate: plants susceptible to the pathogen were also susceptible to the heteropolysaccharides, and vice versa.

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Published

2015-10-22

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