Mechanical sap transmission of a closterovirus from <i>in vitro</i> shoot tip cultures of a leafroll-affected grapevine to <i>Nicotiana benthamiana</i>

Authors

  • P. L. Monette
  • Sharon E. Godkin
  • D. James

DOI:

https://doi.org/10.5073/vitis.1990.29.49-55

Keywords:

virosis, leaf roll, closterovirus, GVA, tissue culture, test plant, transmission, analysis, serology, ISEM, ELISA, etiology

Abstract

Transmission manuelle d'un clostérovirus au Nicotiana benthamiana à partir de cultures in vitro d'une vigne atteinte de l'enroulement

Des cultures in vitro d'une vigne de Vitis vinifera Limberger atteinte de l'enroulement ont été broyées dans une solution tampon contenant de la nicotine. Les extraits ont été inoculés sur des feuilles de Nicotiana benthamiana et de 6 autres espèces de plantes herbacées. 3 semaines plus tard, seuls les plants de N. benthamiana ont démontré des symptômes. Ceux-ci consistaient d'un nanissement systémique accompagné d'un éclaircissement des nervures qui se transformait en chlorose interveinale. Les feuilles de ces N. benthamiana contenaient le virus A de la vigne (GVA), démontré par la méthode ISEM.

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Published

2015-10-23

Issue

Vol. 29 No. 1 (1990): Vitis

Section

Article

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