Double-stranded RNA from rupestris stem pitting-affected grapevines

Authors

  • P. L. Monette
  • D. James
  • Sharon E. Godkin

DOI:

https://doi.org/10.5073/vitis.1989.28.137-144

Keywords:

disease, rupestris stem pitting, virus, dsRNA, variety of vine, in vitro culture, analysis, test plant

Abstract

Nucleic acids were extracted from in vitro shoot tip cultures of 31 grapevine cultivars affected with rupestris stem pitting (RSP) disease and from cultures of 11 RSPfree cultivars, 4 of which were disease-free, 2 of which were fleck-affected and 5 of which were grapevine leafroll disease-affected. Analysis of the extracts by polyacrylamide slab gel electrophoresis showed that 21 of the 31 RSP-affected cultivars contained a previously unreported nucleic acid which was absent from the RSP-free controls. Nuclease digestions showed that the nucleic acid was double-stranded RNA (dsRNA). The apparent size of the dsRNA was inconsistent with that expected for either a viroid or a closterovirus. The observation that 10 of the 31 RSP-affected cultivars lacked this dsRNA is consistent with the view that there may be more than one 'RSP-like' disease. The dsRNA detected in this investigation may be associated with one of these diseases.

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Published

2015-12-15

Issue

Vol. 28 No. 3 (1989): VITIS

Section

Article

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