Caractérisation biochimique des cépages de <i>Vitis vinifera</i> L. par électrophorèse d'isoenzymes foliaires: <p>Essai de classification des variétés </p>

Authors

  • M. Benin
  • J. Gasquez
  • A. Mahfoudi
  • R. Bessis

DOI:

https://doi.org/10.5073/vitis.1988.27.157-172

Keywords:

ampelography, variety of vine, leaf, enzyme, protein, analysis, statistics

Abstract

Biochemical characterization of Vitis vinifera L. cultivars by electrophoresis of leaf isoenzymes:
An attempt to classify grapevine varieties

A number of 40 Vitis vinifera varieties commonly grown in France could be characterized by disc-electrophores is of isoenzymes. Starting from forced cuttings, this technique allows to ana!yze a large number of plants at any time of the year; only low quantities of leaf material are needed and no enzyme purification is necessary. Among 39 bands identified from 3 enzyme systems, 31 were variable: 19, 8 and 4 isoenzymes, respectively, of a-esterase (a-EST), glutamate oxaloacetate transaminase (GOT) and acid phosphatase (PHA).
Combinations of these bands allowed to characterize each variety. 13 groups of rela ted varieties could be established by multivariate analysis of the presence versus absence of bands. The 7 best characterized groups gather varieties which, according to ampelographic classification, are grouped in the same way. The interrelations between isoenzymes and certain ampelographic characters or the presumed geographic origin of the varieties allow to propose a statistical interpretation of the enzymatic classification.

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Published

2015-12-16

Issue

Vol. 27 No. 3 (1988): VITIS

Section

Article

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